Manipulating neuronal activity in behaving rodents
Optogenetics combines genetic engineering and optics to observe and control the function of genetically targeted groups of cells in intact animals. It is used:
- To probe defined neuronal circuits or precise neuronal populations
- To manipulate neuronal circuits in a reversible manner with high spatial and temporal precision (milliseconds timescale)
- To delineate the function and plasticity of defined neuronal circuits during behavior, in combination with electrophysiological and pharmacological approaches
Monitoring neuronal activity in behaving rodents
- simultaneous extracellular recording of the spiking activity of large numbers of individual neurons in different neuronal structures using electrophysiological approaches
- fibred confocal fluorescence microscopy (Cellvizio®) which allows dynamic and functional imaging of neuronal, but also vascular, ensembles in deep brain structures
To perform large scale electrophysiological recordings, large scale cellular/vascular imaging, optogenetics, and pharmacological manipulations, we have developed two versions of versatile cutting-edge commutators:
- A combined mode commutator, for simultaneous use of electrophysiological, optogenetics and pharmacological approaches in behaving rodents.
- A single-mode commutator, for simultaneous use of optogenetics or cellular/vascular imaging and pharmacological approaches in behaving rodents.