Université de BordeauxCluster of excellence



Study and manipulate neuronal activity in behaving rodents

Manipulating neuronal activity

 Optogenetics combines genetic engineering and optics to observe and control the function of genetically targeted groups of cells in intact animals. It is used:

  • To probe defined neuronal circuits or precise neuronal populations
  • To manipulate neuronal circuits in a reversible manner with high spatial and temporal precision (milliseconds timescale)
  • To delineate the function and plasticity of defined neuronal circuits during behavior, in combination with electrophysiological and pharmacological approaches

To perform large scale electrophysiological recordings, large scale cellular/vascular imaging, optogenetics and pharmacological manipulations, we have developed two versions of versatile cutting-edge commutators:

  • A combined mode commutator, for simultaneous use of electrophysiological, optogenetics and pharmacological approaches in behaving rodents.
  • A single mode commutator, for simultaneous use of optogenetics or cellular/vascular imaging and pharmacological approaches in behaving rodents.

Monitoring neuronal activity

  • simultaneous extracellular recording of the spiking activity of large numbers of individual neurons in different neuronal structures using electrophysiological approaches
  • fibred confocal fluorescence microscopy (Cellvizio®) which allows dynamic and functional imaging of neuronal, but also vascular, ensembles in deep brain structures

Develop innovative behavioral models

Morphing Context for Fear Conditioning

The Morphing context apparatus allows to record neurophysiologic activity while leaving the rodent undisturbed as it discriminates between fearful and non-fearful contexts. See video for more detail