Save the date : Training in endomicroscopy in vivo-Use of the Cellvizio system
Study of cerebral vascular network by endomicroscopy in vivo.
Theoretical and practical training in the use of Cellvizio system
June 13-14, 2017
This training will address the theoretical and practical aspects of studying the cerebrovascular network in vivo. This hands-on course will address in real-time visualization and measurement of the vascular reactivity and permeability of the blood-brain barrier in rodent models. The proposed method will be the endomicroscopy in vivo using Cellvizio technology developed by MaunaKea Technologies. During the practical workshops, students will be able to apply this technology to study changes of both the reactivity of cerebral arteries and the sealing of the blood-brain barrier in mice.
The training will focus on the cerebral vascular network, but the applications of this technology are multiple since it is based on tracking fluorescent signals and allows their longitudinal tracking. Thus, it can concern the peripheral circulation, but also the measurement of neuronal activity, including in vigil animals. This training may therefore be of major interest to neuroscientists, neurologists and cardiovascular specialists.
This 2-days workshop will cover :
- Theoretical courses on endomicroscopy in vivo: principles, applications and potentials in the context of neurosciences and cardiovascular research
- Hands on courses will include :
- Surgical implantation of fiber guides (Certification in experimental surgery required for this course)
- Measurement of the blood-brain barrier effectiveness by endomicroscopy in vivo in real time
- Measurement of pial arteries vasoreactivity by endomicroscopy in vivo in real time
- Results analysis and discussion
Selection: Due to space limitation, only 6 participants will be enrolled in each course.
Attendees: Postdoctoral students and researchers with knowledge in fluorescence principles, microscopy, cerebral vascular network and clinical and ethical concepts.